Figure 6

Liver damage induced by α-GalCer and inhibition of BMP/SMAD signaling pathway. Wild-type mice were injected with vehicle (indicated by the gray area across the graphs) or 100 μg/Kg body weight of α-GalCer. (A) Liver alanine aminotransferase levels. (B) Bmp6 and Smad7 mRNA expression in the liver. (C) Liver nuclear extracts analyzed by western blotting. Upper blots: phosphorylated SMAD1/5/8 (pSMAD1/5/8) and total SMAD1/5/8; Lower blots: phosphorylated STAT3 (pSTAT3) and total STAT3.The full-length blots are presented in Supplementary Fig. 6. Data in (A) and (B) are presented as mean ± SEM for a minimum of n = 12 mice per time point. Statistical analysis was performed with one-way ANOVA. *^,†P < 0.01, compared to mice injected with vehicle at each time point.