Figure 4
From: NADPH diaphorase detects S-nitrosylated proteins in aldehyde-treated biological tissues
Typical examples of diformazan production produced by the addition of 1 M NaOH to solutions of NBT + β-NADPH (top panel), NBT + β-NADPH + S-nitrosocysteine (SNC) (middle panel), and β-NADPH + SNC) followed by paraformaldehyde (0.1%, final concentration), NBT and then NaOH (bottom panel). The final concentrations of these agents are those described in Table 1. Note that the initial spikes in absorbance are due to the opening of the cuvette chamber.
