Figure 8
From: NADPH diaphorase detects S-nitrosylated proteins in aldehyde-treated biological tissues
Direct detection of S-nitrosothiols in endothelial vesicles. (A) Vesicle membranes were lysed by sonication and the luminal contents and were examined spectrophotometrically. The lower spectrum is the same sample as the upper spectrum diluted seven-fold in water. (B) Typical example of NO-release from vesicle contents by ultra-violet (UV) light before and after addition of HgCl2. (C) Summary of NO-release from vesicle contents by ultra-violet (UV) light before and after addition of HgCl2. The data are mean ± SEM of 5 separate experiments. *P < 0.05, NO release upon exposure to UV light after addition of HgCl2 (UV2) as compared to before addition of HgCl2 (UV1). HgCl2 exposure abolishes NO release from vesicles.
