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Figure 1

From: Local environment effects on charged mutations for developing aggregation-resistant monoclonal antibodies

Figure 1

(a, b) Structures of the Fv fragments comprising variable heavy (V\(_{\mathrm{H}}\)) and light (V\(_{\mathrm{L}}\)) domains of (a) the wild-type mAb1 (PDB entry 3RU8) and (b) the wild-type mAb2 (modeled based on PDB entry 1DFB). The mutation sites studied in the present work are indicated by stick representations and green labels. Heavy and light complementarity-determining regions (CDRs) are colored blue and red, respectively. (c) Structure of the single-domain (\(V_{\mathrm{H}}\)) antibody A\(\beta\)18-27 dAb (modeled based on PDB entry 3B9V). In the “wild type” dAb, three Ala residues (stick representations colored green) are substituted to the mutation sites (enclosed by a green dashed oval) located at the N-terminus of the third CDR (CDR3). PyMOL version 1.8.2 (https://pymol.org) was used to generate protein figures.

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