Figure 4
(a–c) Residue-wise decomposition of \(\Delta G_{\mathrm{solv}}\) for (a) E10G/D73N/A76K, (b) D60S/E80Q, and (c) E10G/D73N/A76K/D60S/E80Q mutants of mAb1 (blue, red and black colors refer to positively charged, negatively charged and neutral residues, respectively). The mutation sites are indicated by the green arrows. Charged residues that exhibit pronounced variations in individual single-point mutations (taken from Fig. 2 and Supplementary Fig. S1) are represented by the solid arrows labeled with respective mutations. When those charged residues are deleted upon multipoint mutations, the solid arrows are replaced by the dashed ones.
