Figure 3

Pseudopalisade formation. U-251 MG cells at \(4\times 10^6\) cells/mL were cultured within the microdevice. To mimic thrombotic conditions, medium flow was enabled to flow only through the right microchannel. Under unrestricted conditions, medium was refreshed once a day, through both lateral microchannels (modified from previous work45). Scale bar is \(400 \; \mu \, \mathrm {m}\).