Figure 5

M52Q mutation perturbs CaM’s N-terminal domain in the absence of Ca²⁺. Combined ¹H/¹⁵N chemical shift perturbations for M52Q-CaM in the absence and presence of Ca²⁺, relative to WT-CaM based on [¹H, ¹⁵N] HSQC spectra. The dashed line represents two standard deviations (0.09) within the data. Asterisks indicate missing peaks or unassigned residues. NMR Spectra were analyzed using NMRFAM-Sparky⁴⁶.