Table 2 Genetic diversity estimates of 106 by multiplex PCRs (A-M).
MultiplexA | ||||||||||||
Locus | L | Motif | N | k | Range | Ho | He | PIC | NE-PP | F(N) | HW | NA& |
SSeneg4374 | B | Tetra | 61 | 10 | 96–162 | 0.53 | 0.72 | 0.69 | 0.28 | 0.16 | ns | ns |
SSeneg5202 | B | Tetra | 63 | 16 | 210–270 | 0.89 | 0.88 | 0.86 | 0.09 | − 0.01 | ns | ns |
SSeneg16258 | G | Tetra | 63 | 4 | 88–104 | 0.48 | 0.51 | 0.47 | 0.54 | 0.02 | ns | ns |
SSeneg12137 | G | Tetra | 63 | 7 | 141–159 | 0.62 | 0.64 | 0.61 | 0.37 | 0.00 | ns | ns |
SSeneg6381 | G | Tetra | 63 | 18 | 200–266 | 0.71 | 0.87 | 0.85 | 0.10 | 0.10 | (*) | Yes |
SSeneg16050 | Y | Tetra | 63 | 10 | 142–184 | 0.49 | 0.62 | 0.59 | 0.38 | 0.12 | ns | Yes |
SSeneg11269 | Y | Di | 63 | 33 | 183–263 | 0.95 | 0.96 | 0.95 | 0.02 | 0.00 | ns | ns |
SSeneg162554 | R | Tetra | 63 | 9 | 86–118 | 0.89 | 0.80 | 0.76 | 0.21 | − 0.06 | ns | ns |
SSeneg12054 | R | Tetra | 63 | 6 | 159–179 | 0.78 | 0.75 | 0.70 | 0.29 | − 0.03 | ns | ns |
SSeneg3041 | R | Tetra | 63 | 14 | 207–287 | 0.52 | 0.78 | 0.75 | 0.22 | 0.19 | * | Yes |
MultiplexB | ||||||||||||
SSeneg5772 | B | Tetra | 51 | 11 | 80–130 | 0.77 | 0.81 | 0.78 | 0.19 | 0.03 | (*) | ns |
SSeneg12300 | B | Tetra | 51 | 5 | 177–193 | 0.67 | 0.61 | 0.53 | 0.51 | − 0.06 | ns | ns |
SSeneg6326 | B | Tetra | 51 | 9 | 231–267 | 0.82 | 0.80 | 0.77 | 0.19 | − 0.02 | ns | ns |
SSeneg6982 | G | Penta | 51 | 2 | 94–100 | 0.28 | 0.27 | 0.23 | 0.81 | − 0.02 | ns | ns |
SSeneg827 | Y | Tetra | 51 | 6 | 91–111 | 0.55 | 0.63 | 0.58 | 0.41 | 0.07 | ns | ns |
SSeneg395 | Y | Penta | 51 | 8 | 241–276 | 0.80 | 0.82 | 0.79 | 0.18 | 0.01 | ns | ns |
SSeneg14931 | R | Tetra | 51 | 7 | 89–113 | 0.69 | 0.77 | 0.73 | 0.26 | 0.06 | ns | ns |
SSeneg2894 | R | Tetra | 51 | 7 | 178–268 | 0.43 | 0.70 | 0.64 | 0.37 | 0.24 | (*) | Yes |
MultiplexC | ||||||||||||
SSeneg12678 | B | Tetra | 46 | 29 | 121–377 | 0.37 | 0.96 | 0.95 | 0.02 | 0.44 | * | Yes |
SSeneg11209 | G | Tetra | 54 | 10 | 94–134 | 0.78 | 0.79 | 0.76 | 0.20 | 0.01 | (*) | ns |
SSeneg433 | Y | Tetra | 54 | 6 | 101–174 | 0.56 | 0.62 | 0.57 | 0.44 | 0.05 | (*) | ns |
SSeneg7919 | Y | Tetra | 53 | 9 | 174–210 | 0.53 | 0.79 | 0.75 | 0.23 | 0.2 | (*) | Yes |
SSeneg1973 | Y | Tetra | 53 | 23 | 249–329 | 0.94 | 0.93 | 0.92 | 0.04 | − 0.01 | (*) | ns |
SSeneg17673 | R | Tetra | 54 | 5 | 116–177 | 0.44 | 0.73 | 0.67 | 0.35 | 0.24 | * | Yes |
SSeneg10308 | R | Tetra | 54 | 13 | 161–239 | 0.87 | 0.90 | 0.88 | 0.08 | 0.01 | ns | ns |
MultiplexD | ||||||||||||
SSeneg1505 | B | Tetra | 57 | 7 | 112–136 | 0.75 | 0.78 | 0.75 | 0.23 | 0.01 | ns | ns |
SSeneg4306 | B | Tetra | 57 | 2 | 204–208 | 0.51 | 0.50 | 0.37 | 0.72 | − 0.01 | ns | ns |
SSeneg10667 | B | Tetra | 54 | 7 | 277–301 | 0.56 | 0.64 | 0.61 | 0.36 | 0.06 | ns | ns |
SSeneg2307 | G | Tetra | 57 | 6 | 134–166 | 0.53 | 0.55 | 0.50 | 0.51 | 0.02 | (ns) | ns |
SSeneg13116 | G | Tetra | 57 | 7 | 199–235 | 0.63 | 0.72 | 0.69 | 0.28 | 0.06 | ns | ns |
SSeneg1201 | Y | Penta | 57 | 13 | 115–180 | 0.40 | 0.83 | 0.81 | 0.16 | 0.35 | * | Yes |
SSeneg4572 | Y | Tetra | 57 | 2 | 207–215 | 0.26 | 0.48 | 0.36 | 0.73 | 0.29 | * | Yes |
SSeneg4065 | R | Tetra | 57 | 10 | 117–161 | 0.86 | 0.84 | 0.82 | 0.14 | − 0.01 | ns | ns |
SSeneg8782 | R | Tetra | 57 | 10 | 200–242 | 0.88 | 0.83 | 0.81 | 0.15 | − 0.03 | ns | ns |
MultiplexE | ||||||||||||
SSeneg5850 | B | Tetra | 50 | 4 | 74–92 | 0.62 | 0.62 | 0.55 | 0.49 | 0 | ns | ns |
SSeneg2473 | B | Tetra | 50 | 4 | 204–216 | 0.70 | 0.56 | 0.46 | 0.61 | − 0.12 | ns | ns |
SSeneg544 | B | Tetra | 50 | 4 | 282–290 | 0.70 | 0.61 | 0.53 | 0.52 | − 0.08 | ns | ns |
SSeneg87 | G | Tetra | 50 | 12 | 106–166 | 0.64 | 0.67 | 0.62 | 0.37 | 0.02 | ns | ns |
SSeneg5828 | G | Tetra | 49 | 7 | 192–224 | 0.55 | 0.48 | 0.46 | 0.52 | − 0.11 | ns | ns |
SSeneg3415 | Y | Tetra | 50 | 8 | 94–132 | 0.56 | 0.64 | 0.60 | 0.39 | 0.06 | (ns) | ns |
SSeneg5919 | Y | Di | 50 | 8 | 204–224 | 0.66 | 0.74 | 0.69 | 0.31 | 0.05 | ns | ns |
SSeneg585 | R | Tetra | 50 | 7 | 103–127 | 0.62 | 0.75 | 0.71 | 0.27 | 0.07 | (*) | Yes |
SSeneg14542 | R | Tetra | 49 | 8 | 202–244 | 0.67 | 0.76 | 0.71 | 0.29 | 0.06 | ns | ns |
MultiplexF | ||||||||||||
SSeneg1411 | B | Tetra | 64 | 3 | 120–128 | 0.22 | 0.25 | 0.23 | 0.78 | 0.05 | ns | ns |
SSeneg3069 | B | Tetra | 63 | 13 | 183–245 | 0.73 | 0.87 | 0.85 | 0.11 | 0.08 | (*) | ns |
SSeneg9009 | B | Tetra | 64 | 19 | 286–368 | 0.89 | 0.93 | 0.92 | 0.04 | 0.02 | ns | ns |
SSeneg437 | G | Tetra | 65 | 9 | 219–249 | 0.52 | 0.81 | 0.78 | 0.19 | 0.22 | ns | Yes |
SSeneg247 | Y | Tetra | 61 | 7 | 85–122 | 0.71 | 0.72 | 0.68 | 0.30 | − 0.02 | ns | ns |
SSeneg73 | Y | Di | 65 | 16 | 199–255 | 0.85 | 0.86 | 0.84 | 0.12 | 0.01 | ns | ns |
SSeneg12624 | Y | Penta | 64 | 11 | 311–359 | 0.84 | 0.82 | 0.79 | 0.17 | − 0.02 | ns | ns |
SSeneg12095 | R | Tetra | 65 | 4 | 148–160 | 0.51 | 0.48 | 0.42 | 0.61 | − 0.03 | ns | ns |
SSeneg582 | R | Di | 62 | 18 | 224–308 | 0.94 | 0.87 | 0.85 | 0.11 | − 0.05 | ns | ns |
MultiplexG | ||||||||||||
SSeneg3683 | B | Tetra | 69 | 11 | 125–167 | 0.75 | 0.82 | 0.80 | 0.15 | 0.04 | ns | ns |
SSeneg5713 | B | Di | 65 | 21 | 227–311 | 0.86 | 0.88 | 0.87 | 0.08 | 0.01 | ns | ns |
SSeneg1667 | G | Di | 69 | 24 | 225–319 | 0.80 | 0.89 | 0.88 | 0.07 | 0.05 | (ns) | Yes |
SSeneg2891 | Y | Tetra | 68 | 9 | 150–190 | 0.65 | 0.82 | 0.79 | 0.19 | 0.11 | * | Yes |
SSeneg45 | Y | Tetra | 69 | 5 | 242–258 | 0.59 | 0.65 | 0.59 | 0.44 | 0.04 | (*) | ns |
SSeneg12417 | R | Di | 69 | 9 | 199–225 | 0.86 | 0.78 | 0.74 | 0.25 | − 0.06 | (ns) | ns |
SSeneg10524 | R | Tetra | 69 | 7 | 266–286 | 0.75 | 0.71 | 0.66 | 0.34 | − 0.05 | ns | ns |
MultiplexH | ||||||||||||
SSeneg4608 | B | Tetra | 71 | 4 | 82–104 | 0.13 | 0.16 | 0.15 | 0.86 | 0.14 | ns | ns |
SSeneg2868 | B | Tetra | 71 | 9 | 112–172 | 0.78 | 0.83 | 0.80 | 0.17 | 0.03 | (*) | ns |
SSeneg11316 | B | Tetra | 71 | 10 | 214–292 | 0.72 | 0.71 | 0.67 | 0.33 | 0.00 | (*) | ns |
SSeneg287 | G | Tetra | 71 | 7 | 68–114 | 0.55 | 0.52 | 0.47 | 0.55 | − 0.05 | * | ns |
SSeneg90 | G | Tetra | 71 | 13 | 133–175 | 0.93 | 0.85 | 0.84 | 0.13 | − 0.05 | ns | ns |
SSeneg2596 | Y | Tetra | 71 | 5 | 78–104 | 0.38 | 0.40 | 0.35 | 0.68 | 0.00 | * | ns |
SSeneg8412 | Y | Tetra | 71 | 8 | 138–172 | 0.41 | 0.47 | 0.43 | 0.57 | 0.05 | (*) | ns |
SSeneg6827 | R | Tetra | 71 | 4 | 88–100 | 0.32 | 0.33 | 0.30 | 0.71 | − 0.02 | ns | ns |
SSeneg5412 | R | Tetra | 71 | 7 | 148–216 | 0.41 | 0.46 | 0.43 | 0.57 | 0.05 | * | ns |
MultiplexI | ||||||||||||
SSeneg854 | B | Di | 69 | 6 | 85–95 | 0.52 | 0.60 | 0.53 | 0.50 | 0.07 | ns | ns |
SSeneg5899 | B | Tetra | 69 | 5 | 164–216 | 0.41 | 0.47 | 0.43 | 0.58 | 0.06 | ns | ns |
SSeneg5346 | B | Di | 68 | 43 | 184–542 | 0.87 | 0.95 | 0.94 | 0.02 | 0.04 | (*) | ns |
SSeneg1669 | G | Tetra | 69 | 16 | 94–168 | 0.75 | 0.82 | 0.80 | 0.15 | 0.05 | ns | ns |
SSeneg7074 | G | Tetra | 69 | 6 | 144–182 | 0.64 | 0.76 | 0.71 | 0.30 | 0.08 | ns | Yes |
SSeneg4382 | Y | Tetra | 64 | 5 | 92–108 | 0.22 | 0.42 | 0.37 | 0.65 | 0.31 | * | Yes |
SSeneg53551 | Y | Tetra | 67 | 8 | 142–184 | 0.72 | 0.72 | 0.68 | 0.30 | 0.00 | ns | ns |
SSeneg3978 | R | Tetra | 69 | 7 | 84–108 | 0.67 | 0.68 | 0.64 | 0.34 | 0.00 | ns | ns |
SSeneg15332 | R | Tetra | 68 | 19 | 168–250 | 0.91 | 0.89 | 0.87 | 0.08 | − 0.02 | ns | ns |
MultiplexJ | ||||||||||||
SSeneg17159 | B | Tetra | 58 | 5 | 75–93 | 0.48 | 0.47 | 0.42 | 0.59 | − 0.03 | ns | ns |
SSeneg9042 | B | Tetra | 56 | 19 | 174–260 | 0.77 | 0.86 | 0.83 | 0.12 | 0.06 | ns | ns |
SSeneg1723 | G | Tetra | 58 | 7 | 97–127 | 0.55 | 0.51 | 0.47 | 0.53 | − 0.08 | ns | ns |
SSeneg348796 | Y | Tetra | 58 | 6 | 81–101 | 0.78 | 0.67 | 0.61 | 0.41 | − 0.09 | (ns) | ns |
SSeneg7987 | Y | Di | 58 | 32 | 238–354 | 0.88 | 0.94 | 0.93 | 0.03 | 0.03 | (*) | Yes |
SSeneg3077 | R | Tetra | 58 | 4 | 94–110 | 0.40 | 0.36 | 0.33 | 0.68 | − 0.07 | (ns) | ns |
SSeneg10804 | R | Tetra | 54 | 23 | 261–525 | 0.93 | 0.87 | 0.85 | 0.10 | − 0.04 | ns | ns |
MultiplexK | ||||||||||||
SSeneg2083 | B | Tetra | 62 | 9 | 92–124 | 0.69 | 0.65 | 0.61 | 0.36 | − 0.05 | ns | ns |
SSeneg4083 | B | Tetra | 63 | 6 | 220–242 | 0.56 | 0.58 | 0.54 | 0.43 | 0.00 | (ns) | ns |
SSeneg171 | G | Tetra | 63 | 7 | 136–172 | 0.78 | 0.75 | 0.71 | 0.28 | − 0.03 | ns | ns |
SSeneg2487 | G | Tetra | 50 | 26 | 188–328 | 0.98 | 0.95 | 0.93 | 0.03 | − 0.02 | * | ns |
SSeneg566 | Y | Tetra | 63 | 7 | 114–136 | 0.84 | 0.77 | 0.73 | 0.27 | − 0.05 | ns | ns |
SSeneg6876 | R | Tetra | 63 | 21 | 108–198 | 0.94 | 0.91 | 0.90 | 0.06 | − 0.02 | ns | ns |
SSeneg4081 | R | Tetra | 61 | 19 | 268–374 | 0.90 | 0.88 | 0.87 | 0.08 | − 0.02 | ns | ns |
MultiplexL | ||||||||||||
SSeneg7666 | B | Di | 46 | 21 | 162–224 | 0.89 | 0.92 | 0.90 | 0.05 | 0.01 | ns | ns |
SSeneg4003 | B | Di | 46 | 21 | 244–332 | 0.89 | 0.93 | 0.91 | 0.05 | 0.01 | ns | ns |
SSeneg5891 | G | Tetra | 46 | 12 | 97–159 | 0.76 | 0.78 | 0.75 | 0.22 | 0.01 | (ns) | ns |
SSeneg774 | G | Tetra | 46 | 4 | 172–178 | 0.17 | 0.27 | 0.26 | 0.75 | 0.26 | * | Yes |
SSeneg6689 | Y | Tetra | 44 | 5 | 111–131 | 0.11 | 0.41 | 0.38 | 0.61 | 0.55 | (*) | Yes |
SSeneg1147 | Y | Tetra | 46 | 14 | 204–252 | 0.80 | 0.91 | 0.89 | 0.06 | 0.06 | ns | Yes |
SSeneg14333 | R | Tetra | 46 | 8 | 132–172 | 0.37 | 0.83 | 0.79 | 0.18 | 0.38 | * | Yes |
SSeneg2996 | R | Tetra | 45 | 14 | 229–291 | 0.64 | 0.90 | 0.88 | 0.07 | 0.16 | (*) | Yes |
MultiplexM | ||||||||||||
SSeneg506 | B | Tetra | 63 | 6 | 88–114 | 0.22 | 0.66 | 0.60 | 0.43 | 0.49 | * | Yes |
SSeneg387243 | B | Tetra | 62 | 17 | 250–316 | 0.86 | 0.87 | 0.85 | 0.11 | 0.01 | ns | ns |
SSeneg10877 | G | Tetra | 63 | 12 | 177–223 | 0.71 | 0.80 | 0.77 | 0.19 | 0.04 | * | ns |
SSeneg14597 | G | Tetra | 62 | 13 | 250–356 | 0.76 | 0.90 | 0.89 | 0.07 | 0.08 | * | Yes |
SSeneg4328 | Y | Tetra | 63 | 16 | 96–168 | 0.92 | 0.92 | 0.90 | 0.06 | − 0.01 | (ns) | ns |
SSeneg4039 | Y | Di | 60 | 26 | 248–322 | 0.43 | 0.91 | 0.90 | 0.05 | 0.36 | * | Yes |
SSeneg1988 | R | Tetra | 62 | 2 | 91–95 | 0.02 | 0.02 | 0.02 | 0.98 | 0.00 | ns | na |
