Figure 1

Mapping of a flash evoked response by wide-field calcium imaging before and after the optic nerve crush. Upper panel represents the timeline of the experiments. (A) Representation of the mouse head and brain showing the cortical regions of interest related to vision (in green, V1, A, AM, PM, RS) or non-visual regions (in dark blue, sensorimotor areas, color, M1, HL; in light blue, prefrontal areas, AC; red, auditory cortex—not assessed) used to measure the neuronal activity by calcium imaging before and after partial optic nerve crush (ONC). (B) Illustration of the behavioral assessment of the tracking reflex in response to a drifting pattern as shown by two consecutive photographs of the mice. The arrow represents the drifting direction, the tracking reflex is shown by a movement of the head. More explanation and video can be found on the cerebral mechanics website, http://cerebralmechanics.com/products/optomotry-hd-a-virtual/tracking-video-2.html. (C) Illustration of the behavioral assessment of depth avoidance with the visual cliff test in which the time spent by the mouse in the shallow vs deep end of an open field was recorded. (D) Timeline of the experiment. After the implantation of an imaging chamber and habituation to the imaging setup, two baseline and eight post-ONC calcium imaging measurements were performed (after 1 h or at day − 2, 1, 3, 5, 7, 14, 23, 31) during the spontaneous recovery period. CaI Calcium Imaging; V1 primary visual area; A, PM, AM anterior, posterior medial, and anterior medial secondary visual areas; RS restrosplenial cortex; AC anterior cingulate cortex; M1 primary motor cortex; HL Hindlimb sensory cortex. Lower panels represent the temporal decourt maps. (E) Maps at day − 2 (d-2), day 1 (d1), and at day 5 (d5). One frame correspond to 33 ms. The evoked response is contralateral to the LED flash. A round optical chamber was placed over both hemispheres over a denuded skull and a coverslip was added. Calcium imaging was performed through a CCD camera in head-fixed awake Thy1-GCAMP6s mice. (F) The panels to the right shows the amplitude vs time graphs in the left and right visual cortex (V1). (G) Lower panel shows the contralateral response amplitude to a flash in the sane (control, left panel) or injured (ONC, right panel) eye during the spontaneous recovery period. (*) Indicates Wilcoxon test, between baseline and current day and which provided p values under 0.05.