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Figure 1

From: Integrative microRNA and gene expression analysis identifies new epigenetically regulated microRNAs mediating taxane resistance in ovarian cancer

Figure 1

MiRNA profiling of taxol responsive (KF-28) and resistant (KF-Tx) OC cells. (A) Confirmation of chemoresistance of KF-Tx cells to PTX. The figure shows the differential response to PTX and the ability of the epigenetic modulators to restore sensitivity to PTX. (B) Heat map showing differentially expressed miRNAs in the PTX responsive, KF-28, cells versus PTX-resiatnt, KF-Tx, cells, and in KF-Tx cells versus KF-Tx cells following tratement with 5′AZA and TsA. (C) QPCR analysis result of the selected micorRNAs to confirm their downregulation in KF-Tx cells. (D) Relative expression of the same set of miRNAs in KF-Tx cells before and after co-treatment with 5′AZA (10 µM; 3 days) and TsA (300 nM; one day). U6 was used as a control. Values in A, C and D are means ± SE from tree independent experiments. One way anlysis of variance (ANOVA) was performed to get the satsitical significance of diffrence at each point (*p < 0.05, **p < 0.01 and ***p < 0.001).

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