Figure 6

Overexpression of the epigenetically regulated miRNAs restors OC cells’ response to PTX. Quantitative PCR and colony formation assay for KF-Tx cells transfected with (A) micoRNA-204-3p, (B) miRNA-3652, and (C) MiRNA-4286 mimics, respectively. The left panels are qPCR of each gene validating the over-expression while the left panels are colony forming ability results from each miRNA transfected or scrambeled. (D) Viability assay results from A2780-Tx cells after transfection of miRNA-204-3p, miRNA-4286 mimics or scambled RNA, treated with the indicated concentrations of PTX. Viability of the KF-28 (E) and A2780 (F), treated with the indicated concentrations of PTX, after transfection with indicated miRNA inhibitor/s or scramble. (J) Clonogenic assay results of KF-28 cells transfected with the indicated miRNA inhibitor/s or scramble RNA followed by treatment with the indicated concentrations of PTX (left panel) while similar results from A2789 cells (right panel), as the cells were treated with PTX after transfection with the mixture of the indicated miRNAs inhibitors followed by PTX. Results are means ± SE from three independent experiments. One way analysis of variance (ANOVA) was performed to get the statistical significance of difference at each point (*p < 0.05, **p < 0.01 and ***p < 0.001).