Figure 8 | Scientific Reports

Figure 8

From: Integrative microRNA and gene expression analysis identifies new epigenetically regulated microRNAs mediating taxane resistance in ovarian cancer

Figure 8

AKT2 expression mediates the response of KF-28 and KF-Tx and responses to epigentic modulators. (A) Western bloting result shows AKT2 expression in KF-28 and KF-Tx cells in response to PTX at the indicated concentrations. The underlined conc. Indicate the IC50 for each cell line. (B) Western bloting result indicating the downregulation of AKT2 in response to the combination of 5′AZA and TsA in KF-Tx cells. (C) Western bloting result indicating the expression of AKT2 in the KF-28 parental cells transfected with each single miRNA inhibitor, mixture of the three miRNA inhibitors or scrambled RNA together. (D) right panel indicates the downregulation of AKT2 in response to specific siRNA compared with control siRNA in KF-Tx cells. The left panel is annexin/PI results show the relative sensitization of KF-Tx cells to PTX after AKT2 knock down.

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