Figure 4 | Scientific Reports

Figure 4

From: Fused isoselenazolium salts suppress breast cancer cell growth by dramatic increase in pyruvate-dependent mitochondrial ROS production

Figure 4

The effect of fused isoselenazolium salts at 1 μM concentration on mitochondrial respiration (A), flux control factors (B), H2O2 production rate (C) and H2O2/O ratio (D) in permeabilized 4T1 cells. Representative traces of respiration (E) and H2O2 production rate (F) measurement (vehicle—green (E) and light green (F) lines; compound 6—red (E) and purple (F) lines). CI—complex, I; CII—complex II; LEAK—substrate dependent respiration rate; OXPHOS—oxidative phosphorylation dependent state; P—pyruvate; M—malate; ADP—saturating ADP; G—glutamate; S—succinate; Rot—rotenone, AmA—antimycin A. OXPHOS coupling efficiency corresponds to 1-Respiratory Control Ratio-1. Flux Control Factor indicates on the input of each substrate and/or pathway to the electron transfer system performance. Values are shown as mean ± S.D. (n = 3–4 experiments). Significant difference (*- p < 0.05) compared with control.

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