Figure 4

Growth of L. plantarum strain NC8 lacS- and lacA knockouts on GOS. (A) Exemplary growth characteristics of overnight cultures of L. plantarum NC8, NC8ΔlacS or NC8ΔlacA cultured on ½ MRS-C supplemented with 0.5% GOS with optical densities (black bars) and final pH (grey bars). (B) HPAEC-PAD elution patterns of MRS-C + 0.5% GOS without inoculum (black) and spent culture supernatants of NC8 (blue), NC8-ΔlacS (orange) and NC8-ΔlacA (red) grown on ½ MRS-C + 0.5% GOS.