Table 2 Gene targets and respective RT-PCR products used to identify enteric viruses in pig samples.
From: Porcine rotavirus B as primary causative agent of diarrhea outbreaks in newborn piglets
Virus | Viral gene | Primer sequence (5′–3′) | Primer position | RT-PCR product (bp) | References |
|---|---|---|---|---|---|
RVA | VP4 VP7 | Fw-TGGCTTCGCCATTTLATAGACA Rv-ATTTCGGACCATTTATAACC Fw-GGCTTTAAAAGAGAGAATTTCCGTCTGG Rv-GGTCACATCATACAATTCTAATCTAAG | 12–33 868–887 1–21 1038–1062 | 876 1062 | |
RVB | NSP2 | Fw-CTATTCAGTGTGTCG TGAGAGG Rv-CGAAGCGGGCTAGCTTGTCTGC | 1–18 434–451 | 434 | |
RVB | VP7 | Fw-GCGTTGCCACTGCTTCTC Rv-TTTTTATTGGCTTCGGCTACTC | 18–35 796–817 | 800 | |
RVC | VP6 | Fw-GGCTTTAAAAATCTCATTCA Rv-CCTCTAGTTGATTGAACATA | 1–20 251–270 | 270 | |
RVH | VP6 | Fw-ACCAGGTGGAGCAACAAACA Rv-CAGTGCGTGACCAGATCTCA | 529–549 1225–1244 | 716 | |
SDCoV | M | Fw-ATCCTCCAAGGAGGCTATGC Rv-GCGAATTCTGGATCGTTGTT | 67–87 540–560 | 493 | |
TGEV | S | Fw-GTGGTTTTGGTYRTAAATGC Rv-CACTAACCAACGTGGARCTA | 16–35 855–874 | 859 | |
PEDV | S | Fw-TTCTGAGTCACGAACAGCCA Rv-CATATGCAGCCTGCTCTGAA | 1466–1485 2097–2116 | 651 | |
SVV | VP1 | Fw-ACTGACACCGATTTCTCTG Rv-CTAAAGTAAGTGAAACAGGC | 2730–2749 3026–3046 | 316 | |
PEV | 5′ NTR | Fw-CAAGCACTTCTGTTTCCCCGG Rv-GTTAGGATTAGCCGCATTCAGGGG | 197–271 486–509 | 313 |
