Table 1 Cardiomyocyte numbers, body weights, LV mass and dimensions and LV contractile function of 21-day-old C57BL/6 wild-type and Dusp5–/– Mice.

From: DUSP5 expression in left ventricular cardiomyocytes of young hearts regulates thyroid hormone (T3)-induced proliferative ERK1/2 signaling

 

Wild-type

Dusp5–/–

P-value

Mice, n [M/F]

8 [6/2]

8 [4/4]

 

Body weight, g

11.4 ± 0.54

10.7 ± 0.36

0.36

LV mass, mg

39.1 ± 1.6

52.5 ± 1.7

 < 0.001

Cardiomyocyte

Numbers, (millions)

2.36 ± 0.045

2.73 ± 0.065

 < 0.001

LV short-axis parameters

LV mid-papillary level

LVPW-d, mm

0.39 ± 0.018

0.54 ± 0.012

 < 0.001

LVPW-s, mm

0.86 ± 0.038

0.92 ± 0.009

0.15

LVID-d, mm

3.22 ± 0.06

2.87 ± 0.087

0.005

LVID-s, mm

2.39 ± 0.049

1.88 ± 0.056

 < 0.001

Fractional shortening, %

25.9 ± 1

34.2 ± 1.24

 < 0.001

LV mid-apical level

LVPW-d, mm

0.34 ± 0.013

0.53 ± 0.017

 < 0.001

LVPW-s, mm

0.85 ± 0.035

0.93 ± 0.016

0.048

LVID-d, mm

2.71 ± 0.062

2.4 ± 0.087

0.011

LVID-s, mm

1.88 ± 0.049

1.47 ± 0.036

 < 0.001

Fractional shortening, %

30.5 ± 0.94

38.5 ± 1.8

0.0013

LV long-axis parameters

LVPW-d, mm

0.34 ± 0.014

0.54 ± 0.018

 < 0.001

LVPW-s, mm

0.85 ± 0.032

0.93 ± 0.016

0.038

LVID-d, mm

4.29 ± 0.058

4.52 ± 0.052

0.0085

LVID-s, mm

3.49 ± 0.066

3.46 ± 0.047

0.47

Fractional shortening, %

18.5 ± 1.4

23.5 ± 1

0.011

LV volume at diastole, µl

42.1 ± 2.1

34.5 ± 2.2

0.026

LV volume at systole, µl

20 ± 1.1

11.8 ± 0.78

 < 0.001

Ejection fraction, %

52.4 ± 1.4

65.5 ± 1.6

 < 0.001

Stroke volume, µl

22.1 ± 1.37

22.7 ± 1.7

0.79

  1. *C57BL/6 and Dusp5–/– mouse litters were adjusted at P2 to 4 pups/dam). Transthoracic echocardiography, M- and B-modes, were performed in 21-day-old mice under light isoflurane anesthesia. Immediately following this procedure, cardiomyocyte numbers were determined in whole-ventricle cell-suspensions of disaggregated hearts using a hemocytometer. Cardiomyocytes were identified by their shape and size. Data were analyzed by unpaired 2-tailed Students t-test. Values are mean ± SEM. LV left ventricle, LVPWD-d LV posterior wall dimension at end-diastole, LVPWD-s LV posterior wall dimension at end-systole, LVID-d LV internal dimension at end-diastole, LVID-s LV internal dimension at end-systole.
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