Figure 5
From: Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
Nilotinib (Tasigna™) does not affect inducing autophagy in wild-type control mice. (A)–(D) Representative immunoblot for the expression of ATG proteins p62/SQSTM1, LC3B, GABARAPL2 (GL2) from 6, 8, 10 and 12 weeks in cortex, hippocampus, striatum and cerebellum, respectively. β-ACTIN is used as a loading control. (E)–(H) Pooled quantified bar graphs for p62/SQSTM1 for cortex: Age x treatment (F(3,12) = 2.32, p = 0.12), hippocampus: Age x treatment (F(3,12) = 1.87, p = 0.18), striatum: Age x treatment (F(3,12) = 0.99, p = 0.42), and cerebellum: Age x treatment (F(3,12) = 5.40, p = 0.01); (8 weeks: *p < 0.01) respectively. (I)–(L) Pooled quantified bar graphs for LC3B-I for cortex: Age x treatment (F(3,12) = 0.59, p = 0.63), hippocampus: Age x treatment (F(3,12) = 1.72, p = 0.21), striatum: Age x treatment (F(3,12) = 1.13, p = 0.37), and cerebellum: Age x treatment (F(3,12) = 3.06, p = 0.06) respectively. (M)–(P) Pooled quantified bar graphs for LC3B-II for cortex: Age x treatment (F(3,12) = 1.65, p = 0.22), hippocampus: Age x treatment (F(3,12) = 0.57, p = 0.64), striatum: Age x treatment (F(3,12) = 1.22, p = 0.34), and cerebellum: Age x treatment (F(3,12) = 2.22, p = 0.13) respectively. (Q)–(T) Pooled quantified bar graphs for GABARAPL2-II (GL2) for cortex: Age x treatment (F(3,12) = 1.86, p = 0.18); (10 weeks: *p < 0.05), hippocampus: Age x treatment (F(3,12) = 1.01, p = 0.42), striatum: Age x treatment (F(3,12) = 0.08, p = 0.96), and cerebellum: Age x treatment (F(3,12) = 3.74, p = 0.04) respectively. N = 3 (WT-Saline), and (WT-Tasigna) for all the 4 age groups 6, 8, 10, and 12 weeks. Error bars indicate ± SEM. (Mean and ± SEM values are represented in supplementary Tables 4A: Cortex, 4B: Hippocampus, 4C: Striatum, 4D: Cerebellum). N = number of mice. The treatment groups are separated by vertical dashed lines. Statistical analysis was done by Two-way ANOVA followed by Bonferroni post-hoc test. Image brightness and contrast were adjusted for representative purpose. Full uncropped raw blots are represented in the supplementary Fig. S10.
