Figure 6
From: Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
Nilotinib (Tasigna™) is ineffective in inducing autophagy across different stages of disease progression in R6/2. (A)–(D) Representative immunoblot for the expression of ATG proteins p62/SQSTM1, LC3B, GABARAPL2 (GL2) in cortex, hippocampus, striatum and cerebellum respectively from 6, 8, 10 and 12 weeks. β-ACTIN is used as a loading control. (E)–(H) Pooled quantified bar graphs for p62/SQSTM1 for cortex: Age x treatment (F(3,12) = 2.29, p = 0.12), hippocampus: Age x treatment (F(3,12) = 0.28, p = 0.83), striatum: Age x treatment (F(3,12) = 0.39, p = 0.76), and cerebellum: Age x treatment (F(3,12) = 0.27, p = 0.84) respectively. (I)–(L) Pooled quantified bar graphs for LC3B-I for cortex: Age x treatment (F(3,12) = 3.27, p = 0.05), hippocampus: Age x treatment (F(3,12) = 0.27, p = 0.84), striatum: Age x treatment (F(3,12) = 0.85, p = 0.49), and cerebellum: Age x treatment (F(3,12) = 0.55, p = 0.65) respectively. (M)–(P) Summary of quantified bar graphs for LC3B-II for cortex: Age x treatment (F(3,12) = 0.91, p = 0.46), hippocampus: Age x treatment (F(3,12) = 0.13, p = 0.93), striatum: Age x treatment (F(3,12) = 4.68, p = 0.02), and cerebellum: Age x treatment (F(3,12) = 0.43, p = 0.73) respectively. (Q)–(T) Pooled quantified bar graphs for GABARAPL2-II (GL2) for cortex: Age x treatment (F(3,12) = 3.30, p = 0.05), hippocampus: Age x treatment (F(3,12) = 0.20, p = 0.88), striatum: Age x treatment (F(3,12) = 3.09, p = 0.06), and cerebellum: Age x treatment (F(3,12) = 2.24, p = 0.13) respectively. N = 3 (R6/2-Saline), and (R6/2-Tasigna) for all the 4 age groups 6, 8, 10, and 12 weeks. Error bars indicate ± SEM. (Mean and ± SEM values are represented in supplementary Tables 5A: Cortex, 5B: Hippocampus, 5C: Striatum, 5D: Cerebellum). N = number of mice. The treatment groups are separated by vertical dashed lines. Statistical analysis was done by Two-way ANOVA followed by Bonferroni post-hoc test. Image brightness and contrast were adjusted for representative purpose. Full uncropped raw blots are represented in the supplementary Fig. S11.
