Figure 5
From: Intracellular role of IL-6 in mesenchymal stromal cell immunosuppression and proliferation
IL-6 silencing blocks cell division through a decrease of cyclin D1 and ERK1/2phosphorylation. (A) Cyclin D1 expression was analyzed in hMSC-emp or hMSC-IL6i cells treated ( +) or not ( −) with IL-6 (20 ng/ml). GAPDH was used as a loading control (B) hMSC-emp or hMSC-IL6i cells were treated for the indicated times with TNF-α (15 ng/ml) (upper panel) or IL-6 (20 ng/ml) (lower panel) to analyze the level of phospho-ERK1/2. Sam68 was used as a loading control. (C) Cyclin D1 expression in hMSC-emp or hMSC-IL-6i cells treated ( +) or non-treated ( −) with TNF-α (15 ng/ml) for 48 h. GAPDH was used as a loading control.
