Figure 10 | Scientific Reports

Figure 10

From: Epidemiology of a SKin Ulceration Disease (SKUD) in the sea cucumber Holothuria scabra with a review on the SKUDs in Holothuroidea (Echinodermata)

Figure 10

Attempted induction of SKUD in Holothuria scabra via the inoculation of cultured bacteria on the dorsal integument: three cultured bacteria, isolated from SKUD ulcers, were inoculated on artificial scarifications performed on the dorsal integument of the sea cucumbers. (A) Integument scarification/ulceration coverage observed for SKUD-ulcers (purple line) and artificial scarifications (blue line) in SKUD-affected sea cucumbers (controls). (B) Integument scarification/ulceration coverage observed in scarified healthy sea cucumbers (controls). (C) Integument scarification/ulceration coverage observed after inoculation of Vibrio sp. SKUD-MD-20 C1 (culture 1). (D) Integument scarification/ulceration coverage observed after inoculation of Vibrio variabilis SKUD-MD-20 C2 (culture 2). (E) Integument scarification/ulceration coverage observed after inoculation of Vibrio parahaemolyticus SKUD-MD-20 C3 (culture 3) (F) Integument scarification/ulceration coverage observed after inoculation of filtered sea-water (negative controls).

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