Figure 4

Two-color in-resin CLEM of nucleus and endoplasmic reticulum was performed using mWasabi-ER and mCherry2-mito. Thin section (100 nm) of Epon-embedded cells expressing mWasabi-ER (green pseudo color) and mCherry2-mito (red pseudo color) was prepared. Fluorescent images (FM) and electron microscopic images (EM) were obtained as described in Fig. 2. The “Merge” images in (A) and (B) are merged images of a fluorescence image of FM and EM. The images in (B) indicate magnification of images corresponding to the boxed area in the Merge image in (A). The EM images in (C) indicate magnification of image corresponding to the boxed area in the Merge image in (B). Arrowheads in (C) indicate green fluorescence-positive rough endoplasmic reticulum.