Figure 4
From: The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata
(A) Central region of the 2D 1H-15N HSQC spectra of SeviL, showing residues affected by the presence of asialo-GM1. Spectra are coloured by concentration of asialo-GM1 saccharide: 0 mM (black), 0.05 mM (blue), 0.1 mM (cyan), 0.2 mM (green), 0.3 mM (yellow), 0.5 mM (orange) and 1 mM (red). (B) The ribbon diagram of the SeviL dimer, showing the bound saccharide. Residues showing altered HSQC signals in the presence of asialo-GM1 saccharide are coloured blue. (C) Epitope mapping of interactions with GM1b saccharide. The molecular model of the sugar is shown, with the van der Waals surface coloured by STD signal. The residues at each end of the saccharide showed no significant bonding with SeviL. (D) Diagram showing the residues surrounding the sugar ligand. The glucose (bottom left) and sialic acid (top right) residues are highly solvated and make no strong bonds with the protein.
