Table 1 The primer sequences for RT-PCR.
Genes | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
TNF-α (mouse) | CGTCAGCCGATTTGCTATCT | CGGACTCCGCAAAGTCTAAG |
IL-1β (mouse) | TCAGGCAGGCAGTATCACTC | AGCTCATATGGGTCCGACAG |
IL-18 (mouse) | GACAGCCTGTGTTCGAGGATATG | TGTTCTTACAGGAGAGGGTAGAC |
TLR4 (rat) | TACAGTTCGTCATGCTTTCTC | ATTAGGAAGTACCTCTATGCAG |
TLR4 (mouse) | AGCTTCTCCAATTTTTCAGAACTTC | TGAGAGGTGGTGTAAGCCATGC |
MyD88 (rat) | AGGACAAACGAAGGAACTTTT | GCCGATAGTCTGTCTGTTCTAGT |
MyD88 (mouse) | ACCTGTGTCTGGTCCATTGCCA | GCTGAGTGCAAACTTGGTCTGG |
(ZO)-1 (rat) | GCTCACCAGGGTCAAAATGT | GGCTTAAAGCTGGCAGTGTC |
(ZO)-1 (mouse) | GTTGGTACGGTGCCCTGAAAGA | GCTGACAGGTAGGACAGACGAT |
Occludin (rat) | TTACGGCTATGGAGGGTACAC | GACGCTGGTAACAAAGATCAC |
Occludin (mouse) | TGGCAAGCGATCATACCCAGAG | CTGCCTGAAGTCATCCACACTC |
β-Actin (rat) | GTCGTACCACTGGCATTGTG | CTCTCAGCTGTGTGTGTGAA |
β-Actin (mouse) | CTCTCAGCTGTGTGTGTGAA | TGCTGGAAGGTGGACAGTGAGG |
