Figure 4

Bioluminescence imaging (BLI) of PGK-RLuc and Tet-FLuc signals in three representative NSG mice treated with doxycycline (Dox) on (a, b) day 0, (c, d) day 7, or (e, f) day 11 after tumor transplantation. (b, d, f) The simplified 3-compartment model was fitted (dashed black line) to RLuc signal (white circles), shown superimposed with the predicted population of MYC-On cells (blue line), MYC-Off cells (red line), and Escaped cells (green line). Shaded grey region (below 1.60 × 10⁵ photon/s) indicates imaging signal is below the limit of detection. The model-predicted MYC-expressing tumor cell population (orange line) is shown superimposed with Tet-FLuc signal (yellow squares).