Figure 5 | Scientific Reports

Figure 5

From: Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses

Figure 5

Assessment of gonadotrophs in the fetal pituitary at d75 gestation. Representative immunostaining for LHB (brown) in (a) the control fetal male pituitary and (b) the fetal male pituitary after maternal progesterone administration. (c) There was no difference in the area of gonadotroph staining (LHB) in the male fetal pituitary after maternal treatment with vehicle control (Control) or progesterone (Prog) (C = 10; P = 7). (d) Representative dual immunostaining of a control male fetal pituitary showing LHB (green) and PGR (red) showing that the gonadotrophs do not express PGR. (e) Maternal progesterone administration (Prog) decreased PGR expression in the male fetal pituitary (C = 10; P = 7). Box plot whiskers are lowest and highest observed values, box is the upper and lower quartile, with median represented by line in box. Unpaired, two-tailed Student’s t test was used for comparing means of two treatment groups with equal variances accepting P < 0.05 as significant (*P < 0.05).

Back to article page