Figure 3
Quality and quantity of mouse oocytes retrieved at 4 weeks after Samul-tang (SM) administration. (A) Mice were injected with saline (n = 5, Con group) or cyclophosphamide (CP) on days 1, 3, and 5 in the first week; mice injected with CP were then orally administered distilled water (n = 5, CP group) or 5 g/kg SM (n = 5, CP + SM group) five times a week for 4 weeks. Four weeks after SM administration, the mice were superovulated via intraperitoneal injections of 5 IU of pregnant mare serum gonadotropin (PMSG) and 5 IU of human chorionic gonadotropin (hCG) at 48 h after PMSG injection. (B) Oocytes retrieved at 18 h after hCG injection from the Con, CP, and CP + SM mice. (C) Numbers of oocytes, MII oocytes, and MII oocytes with normal chromosomal and spindle alignment, retrieved from the three mouse groups. (D) Areas of oocytes, the ooplasm, and perivitelline space (PVS) in the three mouse groups. Statistical analysis was performed using the Student’s t-test. Scale bars indicate 100 µm. Arrows and arrowheads indicate the days of CP injection and SM administration, respectively. NS not significant.
