Table 2 F-values and the accompanying significance levels from three-way full factorial ANOVA performed to test the effect of the origin of eggs (from wild vs. Captive hens), sample (= eggshell region—background vs. spot), egg status (non-embryonated eggs vs. post-hatched eggshells) and the interaction terms between them on ICP-OES-based eggshell elemental profiles in Black Grouse Tetrao tetrix.

From: Quantifying the functional disparity in pigment spot-background egg colour ICP-OES-based eggshell ionome at two extremes of avian embryonic development

Element

Origin

Sample

Status

Origin × sample

Origin × status

Sample × status

Origin × sample × status

Al

2.22

15.0***

5.08*

2.00

0.38

0.18

4.01*

As

6.59*

5.37*

0.12

1.24

3.35

2.30

0.05

B

12.42**

0.02

23.26***

0.01

11.18**

0.01

0.11

Ba

0.11

0.33

20.84***

0.48

0.40

2.29

0.45

Cd

19.5***

0.44

0.00

0.23

0.02

0.16

0.74

Ce

16.9***

1.39

1.06

3.45

0.002

0.13

0.24

Co

7.07*

3.36

0.09

4.50*

0.001

1.43

0.98

Cr

14.8***

7.83**

4.95*

0.02

6.84*

4.29*

3.52

Cu

5.14*

2.61

0.496

0.820

0.152

0.018

0.003

Fe

13.81***

8.84**

7.63**

4.36*

6.51*

14.62***

10.66**

Ge

1.11

0.04

0.02

0.23

0.40

0.74

1.81

Hf

31.1***

5.84*

5.26*

3.15

8.71**

0.81

1.93

K

0.66

2.49

0.82

2.39

2.30

0.09

2.66

La

11.69**

3.64

0.27

0.04

5.31*

0.01

1.20

Lu

23.15***

2.18

3.46

0.87

7.22*

0.28

2.42

Mg

1.84

0.76

0.00

2.82

4.42*

0.44

3.18

Mn

1.64

0.18

13.60***

0.42

1.59

0.18

0.29

Mo

0.92

0.44

2.25

1.55

0.01

1.89

4.82

Na

0.63

0.38

0.23

2.38

1.91

0.06

3.91

Nd

37.15***

0.32

2.74

1.23

4.66*

0.01

1.57

Ni

7.89**

6.57*

3.15

0.55

4.92*

2.10

4.39*

Pb

23.28***

5.87*

4.62*

0.80

7.56**

0.49

5.34*

Pr

1.73

1.00

1.06

2.49

2.52

0.61

2.74

Rh

3.60

0.00

0.16

2.35

2.80

0.57

1.22

Ru

11.25**

0.00

1.93

0.87

4.29*

0.16

0.01

Sb

1.65

1.46

0.05

0.02

0.09

0.05

0.25

Sc

0.07

2.06

0.03

2.37

0.79

0.00

3.04

Se

9.60**

0.36

1.93

0.32

4.50*

0.20

3.57

Si

7.00*

3.45

0.00

0.32

0.20

0.22

0.75

Ta

4.69*

0.23

0.67

1.06

0.01

0.70

0.08

Tb

0.24

2.16

0.67

1.97

0.73

0.19

2.26

Te

43.97***

1.60

7.47**

2.63

10.94**

3.19

4.19*

Ti

1.20

0.82

0.03

0.44

0.10

0.00

0.00

Tl

9.33**

0.88

1.59

4.10*

1.89

0.00

0.04

Zn

0.94

0.79

0.87

0.43

0.40

0.26

0.12

  1. Statistically significant effects are in bold.
  2. The elemental concentrations corresponding to the tested effects are listed in Table S3.
  3. *P < 0.05; **P < 0.01; ***P < 0.001.
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