Table 1 Geographic locations, sample size (n), and estimates of genetic diversity for the 78 populations of Bufo bufo analysed in this study. h: haplotype diversity; π: nucleotide diversity; He: expected heterozygosity; Ar; allelic richness.
Sample | Location | Latitude | Longitude | mtDNA | Microsatellites | ||||
|---|---|---|---|---|---|---|---|---|---|
(N) | (E) | n | h | π | n | He | Ar | ||
1 | Godz | 45.898 | 13.989 | 5 | 0 | 0 | – | – | – |
2 | Musi | 46.316 | 13.251 | 3 | 0 | 0 | 10 | 0.443 | 2.932 |
3 | Sauris | 46.475 | 12.616 | – | – | – | 8 | 0.505 | 2.834 |
4 | Monte Cesen | 45.946 | 12.012 | – | – | – | 10 | 0.536 | 3.505 |
5 | Monte Baldo | 45.678 | 10.780 | 4 | 0.833 | 0.0032 | 7 | 0.524 | 3.202 |
6 | Endine Gaiano | 45.792 | 10.009 | 1 | – | – | 10 | 0.477 | 3.378 |
7 | Arona | 45.734 | 8.552 | 1 | – | – | 9 | 0.546 | 3.719 |
8 | Borgofranco di Ivrea | 45.511 | 7.873 | 1 | – | – | – | – | – |
9 | Fiano (Torino) | 45.219 | 7.540 | – | – | – | 10 | 0.469 | 2.986 |
10 | Pigna | 44.626 | 7.456 | – | – | – | 9 | 0.552 | 4.056 |
11 | Barge | 44.716 | 7.326 | 1 | – | – | – | – | – |
12 | Cuneo | 44.351 | 7.534 | – | – | – | 1 | – | – |
13 | Thorame Haute | 44.089 | 6.534 | 4 | 0.833 | 0.0457 | 7 | – | – |
14 | Saint–Auban | 43.843 | 6.727 | 5 | 0.600 | 0.0005 | 7 | – | – |
15 | La Martre | 43.797 | 6.598 | 5 | 0.400 | 0.0003 | 7 | 0.446 | 2.561 |
16 | Gattières | 43.759 | 7.174 | 5 | 0.600 | 0.0005 | 7 | 0.390 | 2.512 |
17 | Rocchetta Nervina | 43.883 | 7.603 | 5 | 0.700 | 0.0008 | 10 | 0.457 | 2.970 |
18 | Ceriana | 43.880 | 7.773 | 9 | 0.556 | 0.0273 | 10 | 0.463 | 2.870 |
19 | Molini di Triora | 43.988 | 7.776 | 3 | 1 | 0.0022 | 5 | 0.508 | 3.242 |
20 | Mendatica | 44.072 | 7.811 | 3 | 0 | 0 | 3 | – | – |
21 | Lecchiore | 43.916 | 7.921 | 6 | 0.333 | 0.0003 | – | – | – |
22 | Villanova D'albegna | 44.041 | 8.119 | – | – | – | 9 | 0.491 | 3.233 |
23 | Calice Ligure | 44.203 | 8.287 | 3 | 0.667 | 0.0016 | 10 | 0.557 | 3.371 |
24 | Albisola Superiore | 44.343 | 8.496 | 5 | 0.900 | 0.0078 | 10 | 0.413 | 2.515 |
25 | Brignano-Frascata | 44.829 | 9.038 | 1 | – | – | – | – | – |
26 | Lerma | 44.620 | 8.712 | 4 | 0.500 | 0.0008 | 10 | 0.572 | 3.777 |
27 | San Giorgio a Bavari | 44.428 | 9.011 | 1 | – | – | 9 | 0.458 | 3.143 |
28 | Zavattarello | 44.891 | 9.264 | 1 | – | – | 8 | 0.581 | 3.915 |
29 | Varese Ligure | 44.480 | 9.607 | 3 | 0 | 0 | 10 | 0.411 | 2.965 |
30 | Sarzana Ligure | 44.270 | 9.458 | 1 | – | – | – | – | – |
31 | Cerreto Laghi | 44.303 | 10.244 | 2 | – | – | 10 | 0.48 | 3.331 |
32 | Monte San Pietro | 44.360 | 11.108 | 1 | – | – | 10 | 0.569 | 3.994 |
33 | Monghidoro | 44.248 | 11.346 | 2 | – | – | – | – | – |
34 | Campo Tizzoro | 44.039 | 10.862 | 3 | 0.667 | 0.0016 | – | – | – |
35 | Ciola | 43.983 | 12.130 | 1 | – | – | 10 | 0.507 | 3.622 |
36 | Terranuova Bracciolini | 43.555 | 11.569 | 6 | 1 | 0.0034 | 10 | 0.545 | 3.510 |
37 | Serra San Quirico | 43.428 | 13.044 | 1 | – | – | 9 | 0.49 | 3.518 |
38 | Teramo | 42.687 | 13.721 | – | – | – | 10 | 0.476 | 3.086 |
39 | San Gemini | 42.608 | 12.558 | 5 | 1 | 0.0023 | 10 | 0.476 | 3.288 |
40 | Canale Monterano | 42.140 | 12.097 | 6 | 0.733 | 0.0016 | – | – | – |
41 | Rocca Sinibalda | 42.275 | 12.926 | 3 | 0 | 0 | – | – | – |
42 | Scoppito | 42.361 | 13.265 | 1 | – | – | 10 | 0.556 | 3.860 |
43 | Jenne | 41.890 | 13.171 | 5 | 0.900 | 0.0023 | – | – | – |
44 | Bosco del Foglino | 41.471 | 12.719 | 3 | 1 | 0.0011 | – | – | – |
45 | Fara Filorum Petri | 42.248 | 14.188 | 3 | 1 | 0.0081 | 10 | 0.495 | 3.489 |
46 | Opi | 41.791 | 13.807 | 3 | 1 | 0.0027 | 9 | 0.464 | 3.156 |
47 | Doganella | 41.750 | 12.761 | 4 | 0 | 0 | 9 | 0.580 | 3.854 |
48 | Molella | 41.268 | 13.046 | 4 | 0.500 | 0.0016 | – | – | – |
49 | San Pietro Infine | 41.444 | 13.968 | 3 | 0.667 | 0.0016 | – | – | – |
50 | San marco la Catola | 41.541 | 15.040 | 3 | 0 | 0 | 8 | 0.369 | 2.242 |
51 | Lago Matese | 41.409 | 14.405 | 5 | 1 | 0.0086 | 10 | 0.606 | 4.090 |
52 | Biccari | 41.370 | 15.172 | 3 | 0.667 | 0.0011 | 10 | 0.529 | 3.345 |
53 | Grata | 41.276 | 13.710 | 3 | 0.667 | 0.0075 | 10 | 0.488 | 3.319 |
54 | Camposauro | 41.174 | 14.582 | 5 | 0.800 | 0.0013 | – | – | – |
55 | Tufara | 41.061 | 14.714 | 3 | 0.667 | 0.0011 | 9 | 0.55 | 3.496 |
56 | Spinazzola | 40.998 | 16.059 | 2 | – | – | 7 | 0.573 | 3.951 |
57 | Monticchio | 40.929 | 15.603 | 5 | 0 | 0 | 10 | 0.485 | 2.386 |
58 | Lago Laceno | 40.806 | 15.095 | 8 | 0.607 | 0.0005 | 8 | 0.499 | 3.223 |
59 | Tricarico | 40.618 | 16.145 | 3 | 0.667 | 0.0005 | 9 | 0.499 | 3.158 |
60 | Campora | 40.289 | 15.327 | 3 | 0 | 0 | 10 | 0.541 | 3.625 |
61 | Lago Cessuta | 40.254 | 15.784 | 5 | 0.400 | 0.0003 | 9 | 0.537 | 3.865 |
62 | Contrada Massadita | 39.942 | 15.806 | 4 | 0.833 | 0.0008 | – | – | – |
63 | Orsomarso | 39.800 | 15.908 | 3 | 0 | 0 | 10 | 0.43 | 2.627 |
64 | Lago Farneto | 39.664 | 16.157 | 5 | 0.400 | 0.0003 | 10 | 0.354 | 2.789 |
65 | Fagnano Castello | 39.556 | 16.021 | 2 | – | – | 8 | 0.476 | 3.232 |
66 | Macchialonga | 39.339 | 16.584 | – | – | – | 9 | 0.431 | 2.793 |
67 | Fiumefreddo Bruzio | 39.225 | 16.072 | 5 | 0 | 0 | 8 | 0.412 | 3.155 |
68 | Falerna | 39.002 | 16.174 | 1 | – | – | – | – | – |
69 | Lago dell'Angitola | 38.740 | 16.236 | 2 | – | – | 10 | 0.466 | 2.772 |
70 | Stilo | 38.478 | 16.469 | 1 | – | – | 7 | – | – |
71 | Oppido Mamertino | 38.291 | 15.989 | 5 | 0 | 0 | – | – | – |
72 | Gambarie | 38.181 | 15.846 | 2 | – | – | 10 | 0.528 | 3.715 |
73 | Alberobello | 40.780 | 17.254 | 2 | – | – | – | – | – |
74 | Lecce | 40.345 | 18.167 | 5 | 0.400 | 0.0003 | 8 | 0.422 | 2.613 |
75 | Fiume Irminio | 36.929 | 14.674 | 4 | 0.667 | 0.0016 | 7 | 0.398 | 2.840 |
76 | Corleone | 37.869 | 13.305 | 4 | 0.833 | 0.0012 | 10 | 0.411 | 2.897 |
77 | Rosolini | 36.823 | 15.032 | 1 | – | – | – | – | – |
78 | Maletto | 37.853 | 14.831 | 1 | – | – | – | – | – |
231 | 500 | ||||||||
