Figure 4
Serum AgSAA levels in A. gueldenstaedtii challenged with heat-inactivated or live A. hydrophila. Experiment with heat-inactivated bacteria: juvenile Russian sturgeons were ip challenged with heat-inactivated A. hydrophila or sterile PBS (control PBS), and serum AgSAA levels analysed at 1 dpc (a) or 3 dpc (b). Experiment with live bacteria: juvenile Russian sturgeons were ip challenged with live A. hydrophila or sterile PBS (control PBS), and serum AgSAA levels analysed at 3 dpc (c). Individual and median values of serum AgSAA levels (μg/ml) are indicated. Data were transformed to meet normality (using the inverse square root or logarithmic transformation for the challenge with heat-inactivated and live bacteria trial, respectively) and afterwards the one-way ANOVA with post-hoc Tukey´s test was applied for multiple comparisons. Statistical significances (p-value) are shown. The correlation between serum AgSAA levels and relative liver saa mRNA levels (2^-ΔCT, determined using gapdh as housekeeping gene) is shown in (d). Statistical significance (p-value) analysed by Spearman test is indicated. This correlation was also found when saa mRNA levels were determined using act-b as housekeeping gene.
