Figure 5 | Scientific Reports

Figure 5

From: Investigating seafood substitution problems and consequences in Taiwan using molecular barcoding and deep microbiome profiling

Figure 5

Primers for the fish taxonomic identification. (A) The primers in literature were aligned to reference sequences of Reinhardtius hippoglossoides, Gadus chalcogrammus, Gadus macrocephalus and Gadus ogacin in NCBI. These primers manifest poor query coverage (< 70%) to the fish species of interest in this study. (B) Primers and gel images of the amplified DNA. The primers Fish1R and Fish4F/4R were designed using the multiple sequence alignment of the reference sequences of cod, halibut, salmon, swordfish and amberjack. Fish3F and Fish5R were based on tuna and snapper.

Back to article page