Figure 8 | Scientific Reports

Figure 8

From: Selenization of S. cerevisiae increases its protective potential in experimental autoimmune encephalomyelitis by triggering an intestinal immunomodulatory loop

Figure 8

Effect of S. cerevisiae and Selemax on intestinal mucosal inflammatory markers. EAE mice received 14 oral doses of S. cerevisiae or Selemax. The mRNA expression of Tbx21 (a), Rorc (b), Gata3 (c), Foxp3 (d), Tgf (e), Clec7 (f), Tlr2 (g), Tlr4 (h) and Ido (i) were performed in small intestine samples. Gut explants of small intestine were cultured for 6 h and the production of IL-10 (j), IFN-γ (k), TNF-α (l), IL-17A (m), IL-6 (n) and IL-2 (o) were assessed in the supernatants. The gene expression and cytokine levels were used to calculate the fold change in relation to EAE group (p). Statistical analysis was performed by one-way ANOVA and subsequent Tukey’s test (a–o) and two-way ANOVA and subsequent Tukey’s test (p). All data were expressed as the mean ± SD and statistical differences were represented by *p < 0.05 and **p < 0.01; & p < 0.05 between EAE and EAE/Selemax. Two independent experiments were combined, n = 7–8 mice/group. The graphs were created using GraphPad Prism v.8.0.2 and image was modified with Adobe Photoshop v.22.0.0.

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