Figure 5 | Scientific Reports

Figure 5

From: Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

Figure 5

Representative macroscopic images of EVPOMEs at Day 0 (immediately after cells were seeded), Day 4 (transferred into air–liquid interface culture) and Day 11 (completion of EVPOMEs manufacturing). The initial construct size was 20 mm in diameter (Scale bar = 20 mm). Data shown are presented as mean ± SD of diameters of the EVPOMEs (n = 5). The collagen gels with EDC crosslinking showed little contraction as indicated. As p values determined by a paired t test are shown, the EVPOME dimeter was significantly reduced (contracted) after manufacturing the grid-type scaffolds (G-R and G-T) without 1% EDC crosslinking, compared with the crosslinked ones. Although the contraction of other EVPOMEs was not statistically significant, the resulting EVPOME diameters were inconsistent due to large variations.

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