Figure 3
Representative saturation binding curve of [3H]WIN35,428 to DAT on U2OS-DAT membranes. Total binding (●) and non-specific binding (○) were determined in the absence or presence of 100 μM GBR12909. Specific binding (■) was obtained by linear subtraction of non-specific binding from total binding. Data are shown as the mean of a representative graph of three separate experiments each performed in triplicate.
