Table 3 Validation of 28 RNAs extracted from patients attending Broomfield Hospital between April and June 2020.

From: CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19

Sample	Date	Viasure			COV2-ID				Sansure
Sample	Date	ORF1ab	N	IC	Nsp10	N	JUN	EICAS	ORF1ab	N	IC	D614G status
A1	24-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A2	29-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A3	30-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A4	01-05-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	Mutant (G)
A5	01-05-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A6	03-05-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A7	03-05-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
A8	03-05-2020	−ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
B1	14-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	−ve	+ve	+ve	Mutant (G)
B2	26-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	Mutant (G)
B3	26-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	Mutant (G)
B4	26-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	Mutant (G)
B5	27-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	+ve	Mutant (G)
C1	14-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C2	15-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C3	15-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C4	15-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C5	17-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C6	17-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
C7	19-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
D1	13-06-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
D2	27-04-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
D3	13-06-2020	+ve	+ve	+ve	+ve	+ve	+ve	+ve	–	-	-	Mutant (G)
E1	N/A	−ve	−ve	+ve	−ve	−ve	+ve	+ve	−ve	−ve	+ve	N/A
E2	N/A	−ve	−ve	+ve	−ve	−ve	+ve	+ve	−ve	−ve	+ve	N/A
E3	N/A	−ve	−ve	+ve	−ve	−ve	+ve	+ve	–	-	-	N/A
E4	N/A	−ve	−ve	+ve	−ve	−ve	+ve	+ve	−ve	−ve	+ve	N/A
E5	N/A	−ve	−ve	+ve	−ve	−ve	+ve	+ve	−ve	−ve	+ve	N/A
n =	28

The four positive (A-D) and single negative batches were collected at different times. Negative test results are highlighted in bold. The Viasure data were obtained at the hospital, and ten of the samples were screened using a commercial kit (Sansure). The result of the genotyping tests are also shown.

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Table 3 Validation of 28 RNAs extracted from patients attending Broomfield Hospital between April and June 2020.

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