Table 1 The relative composition of lipid classes in extracts of Porosira glacialis, Odontella aurita and Chlorella vulgaris extracted with dichloromethane/methanol (2:1 v/v; DCM/MeOH), hexane/isopropanol (2:1 v/v; Hexane/IPA) and hexane, using three pre-treatments; no treatment (control), shaking at 1000 RPM for 60 min, (mixing), or sonication at 20 kHz for 10 min (sonication).

P. glacialis

TAG

5.66 ± 0.20

5.55 ± 0.18

5.97 ± 0.37

7.92 ± 0.75

7.12 ± 0.42

8.14 ± 0.55

52.61 ± 5.56

46.12 ± 4.38

43.29 ± 3.13

DAG

10.56 ± 0.54

12.25 ± 0.62

17.38 ± 0.77

14.84 ± 0.96

15.64 ± 1.11

19.86 ± 1.57

9.13 ± 0.78

12.19 ± 2.57

20.80 ± 3.33

FFA

9.81 ± 0.70

9.31 ± 2.43

8.67 ± 1.07

12.27 ± 0.75

12.71 ± 1.75

14.02 ± 1.72

37.15 ± 6.28

40.48 ± 6.31

33.72 ± 5.82

MGDG

40.06 ± 1.09

36.30 ± 2.62

26.92 ± 1.84

36.69 ± 1.58

35.56 ± 1.08

33.28 ± 5.07

1.11 ± 0.57

1.21 ± 0.48

2.18 ± 0.28

DGDG

0

0

8.52 ± 0.46

0

0

0

0

0

0

PG

19.10 ± 0.41

21.6 ± 2.32

0

15.95 ± 1.18

14.29 ± 0.86

0

0

0

0

PC

14.81 ± 0.68

14.95 ± 2.19

32.55 ± 1.21

12.33 ± 1.95

14.68 ± 1.17

24.70 ± 2.51

0

0

0

O. aurita

TAG

4.39 ± 0.28

8.27 ± 0.93

7.45 ± 0.41

3.70 ± 0.77

4.23 ± 0.46

5.75 ± 0.93

3.92 ± 0.48

3.59 ± 0.56

5.93 ± 0.61

DAG

3.05 ± 0.14

4.24 ± 0.58

3.58 ± 0.15

3.98 ± 0.62

3.53 ± 0.43

3.78 ± 0.52

3.71 ± 0.43

2.57 ± 0.39

4.39 ± 0.26

FFA

70.81 ± 3.48

71.81 ± 3.79

74.67 ± 1.67

78.26 ± 3.63

80.70 ± 1.91

76.26 ± 4.35

92.37 ± 0.91

93.84 ± 0.92

89.68 ± 0.77

MAG

2.55 ± 0.31

3.14 ± 0.44

2.79 ± 0.31

2.49 ± 0.30

2.64 ± 0.27

2.78 ± 0.40

0

0

0

MGDG

0.39 ± 0.08

0.89 ± 0.16

1.06 ± 0.27

0.54 ± 0.36

0.47 ± 0.21

1.22 ± 0.48

0

0

0

PC

18.81 ± 3.33

11.64 ± 5.04

10.45 ± 1.64

11.03 ± 2.18

8.43 ± 1.13

10.21 ± 4.96

0

0

0

C. vulgaris

TAG

17.42 ± 1.02

15.88 ± 1.49

19.11 ± 0.82

4.09 ± 0.33

9.00 ± 1.38

21.07 ± 2.04

20.87 ± 3.68

39.39 ± 2.08

45.84 ± 2.44

DAG

9.69 ± 0.45

9.38 ± 0.79

10.29 ± 0.51

5.72 ± 0.56

7.81 ± 0.34

11.67 ± 0.94

5.56 ± 1.13

10.18 ± 0.55

15.80 ± 0.72

FFA

39.31 ± 2.21

38.40 ± 2.32

26.33 ± 1.18

54.08 ± 2.53

45.52 ± 2.10

25.06 ± 2.93

58.42 ± 3.66

39.76 ± 2.48

12.40 ± 1.31

MGDG

15.06 ± 1.39

13.76 ± 0.75

19.54 ± 0.78

17.53 ± 2.67

21.27 ± 0.75

22.67 ± 2.10

0

0

3.23 ± 0.42

DGDG

0

0

3.08 ± 0.12

0

0

2.50 ± 0.28

0

0

0

PC

18.52 ± 1.23

22.58 ± 2.75

21.65 ± 1.95

18.57 ± 4.48

16.40 ± 1.72

17.04 ± 1.20

15.15 ± 5.56

10.67 ± 1.24

22.73 ± 3.97