Table 2 The relative content (%) of fatty acids from Porosira glacialis extracted in dichloromethane/methanol (2:1 v/v; DCM/MeOH), hexane/isopropanol (2:1 v/v; hexane/IPA) and hexane, using three pre-treatments; no treatment (control), shaking at 1000 RPM for 60 min, (mixing), or sonication at 20 kHz for 10 min (sonication).

From: Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption

P. glacialis

DCM/MeOH

Hexane/IPA

Hexane

FA

Control

Mixing

Sonication

Control

Mixing

Sonication

Control

Mixing

Sonication

C14:0

4.60 ± 0.42

4.63 ± 0.38

4.94 ± 0.32

4.45 ± 0.10

4.51 ± 0.35

5.30 ± 0.33

4.22 ± 0.12

4.54 ± 0.18

4.38 ± 0.15

C16:0

7.03 ± 3.81

7.33 ± 3.43

5.86 ± 0.57

5.48 ± 0.13

6.67 ± 2.38

5.91 ± 0.31

5.43 ± 0.17

5.70 ± 0.24

6.06 ± 0.12

C16:1n-7

11.16 ± 1.02

11.45 ± 0.84

11.89 ± 0.33

11.89 ± 0.16

11.44 ± 0.45

11.94 ± 0.23

13.43 ± 0.13

13.39 ± 0.42

13.23 ± 0.07

C16:2n-4

3.21 ± 0.56

3.24 ± 0.49

3.48 ± 0.05

3.36 ± 0.06

3.20 ± 0.27

3.40 ± 0.06

2.90 ± 0.02

3.95 ± 2.25

2.86 ± 0.02

C16:3n-4

6.32 ± 1.12

6.42 ± 0.96

6.91 ± 0.11

7.09 ± 0.15

6.87 ± 0.61

7.09 ± 0.15

5.87 ± 0.01

5.78 ± 0.17

5.97 ± 0.06

C16:4n-1

31.38 ± 4.62

30.79 ± 3.32

29.53 ± 0.46

30.32 ± 0.64

30.86 ± 1.75

29.87 ± 0.71

28.28 ± 0.11

27.72 ± 0.75

27.73 ± 0.31

C18:4n-3

4.69 ± 0.73

4.71 ± 0.63

4.93 ± 0.07

4.90 ± 0.10

4.63 ± 0.32

4.94 ± 0.07

5.37 ± 0.02

5.17 ± 0.14

5.09 ± 0.07

C20:5n-3

28.68 ± 5.24

29.18 ± 4.55

30.73 ± 0.51

30.31 ± 0.64

28.47 ± 2.58

29.43 ± 0.43

31.09 ± 0.21

30.99 ± 1.06

30.90 ± 0.15

C22:6n-3

2.93 ± 1.54

2.26 ± 1.81

1.73 ± 1.85

2.20 ± 1.73

3.34 ± 0.26

2.12 ± 1.64

3.41 ± 0.07

2.76 ± 1.40

3.79 ± 0.08

SFA

11.63 ± 4.21

11.96 ± 3.73

10.80 ± 0.88

9.93 ± 0.12

11.19 ± 2.72

11.20 ± 0.61

9.79 ± 0.32

10.24 ± 0.36

10.44 ± 0.25

PUFA

77.21 ± 3.24

76.59 ± 3.09

77.30 ± 1.18

78.19 ± 0.23

77.37 ± 2.28

76.86 ± 0.77

76.78 ± 0.36

76.37 ± 0.77

76.34 ± 0.32

  1. Data shown is averages of replicates ± SD, n = 5.
  2. SFA saturated fatty acids, PUFA polyunsaturated fatty acids.