Table 3 The relative content (%) of fatty acids from Odontella aurita extracted in dichloromethane/methanol (2:1 v/v; DCM/MeOH), hexane/isopropanol (2:1 v/v; hexane/IPA) and hexane, using three pre-treatments; no treatment (control), shaking at 1000 RPM for 60 min, (mixing), or sonication at 20 kHz for 10 min (sonication).

From: Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption

O. aurita

DCM/MeOH

Hexane/IPA

Hexane

FA

Control

Mixing

Sonication

Control

Mixing

Sonication

Control

Mixing

Sonication

C14:0

11.29 ± 0.45

12.15 ± 0.12

12.46 ± 0.29

12.09 ± 0.23

12.26 ± 0.16

12.64 ± 0.15

11.81 ± 0.32

12.12 ± 0.19

12.10 ± 0.12

C16:0

27.14 ± 0.33

26.18 ± 0.18

26.10 ± 0.20

27.49 ± 0.29

27.38 ± 0.27

26.88 ± 0.13

28.84 ± 0.25

28.25 ± 0.24

27.91 ± 0.25

C16:1n-7

36.51 ± 0.65

35.71 ± 0.30

35.68 ± 0.29

37.23 ± 0.14

37.11 ± 0.37

36.06 ± 0.40

37.86 ± 0.26

38.60 ± 0.10

37.31 ± 0.22

C16:2n-4

3.03 ± 0.09

3.10 ± 0.02

3.04 ± 0.03

2.96 ± 0.02

2.96 ± 0.02

2.96 ± 0.04

2.93 ± 0.02

2.83 ± 0.01

2.81 ± 0.04

C16:3n-4

2.28 ± 0.07

2.35 ± 0.01

2.30 ± 0.02

2.02 ± 0.04

2.07 ± 0.03

2.10 ± 0.04

1.80 ± 0.02

1.62 ± 0.01

1.67 ± 0.05

C18:0

1.11 ± 0.03

1.20 ± 0.02

1.25 ± 0.05

1.21 ± 0.12

1.41 ± 0.07

1.53 ± 0.13

1.22 ± 0.07

1.40 ± 0.04

1.62 ± 0.04

C18:1n-9

2.98 ± 0.06

3.11 ± 0.03

3.02 ± 0.02

2.70 ± 0.05

2.77 ± 0.05

2.82 ± 0.06

2.47 ± 0.02

2.29 ± 0.03

2.31 ± 0.08

C18:1n-7

1.60 ± 0.02

1.94 ± 0.02

1.81 ± 0.02

1.63 ± 0.09

1.57 ± 0.10

1.58 ± 0.12

1.13 ± 0.02

1.17 ± 0.01

1.15 ± 0.01

C18:2n-6

1.21 ± 0.05

1.27 ± 0.05

1.25 ± 0.01

1.13 ± 0.01

1.20 ± 0.03

1.20 ± 0.03

1.04 ± 0.04

1.12 ± 0.05

1.13 ± 0.03

C20:5n-3

10.96 ± 0.17

12.35 ± 0.15

11.56 ± 0.12

10.03 ± 0.15

10.17 ± 0.15

11.14 ± 0.09

9.77 ± 0.07

10.11 ± 0.11

10.67 ± 0.20

C22:6n-3

1.89 ± 1.25

0.65 ± 0.72

1.52 ± 0.70

1.52 ± 0.03

1.08 ± 0.64

1.09 ± 0.80

1.14 ± 0.52

0.47 ± 0.45

1.32 ± 0.62

SFA

39.54 ± 0.29

39.53 ± 0.31

39.82 ± 0.34

40.79 ± 0.17

41.05 ± 0.29

41.05 ± 0.27

41.86 ± 0.31

41.77 ± 0.34

41.63 ± 0.39

MUFA

41.09 ± 0.73

40.76 ± 0.31

40.51 ± 0.31

41.56 ± 0.14

41.46 ± 0.31

40.46 ± 0.49

41.46 ± 0.29

42.06 ± 0.11

40.77 ± 0.28

PUFA

19.37 ± 0.99

19.72 ± 0.61

19.67 ± 0.64

17.65 ± 0.19

17.49 ± 0.54

18.50 ± 0.64

16.68 ± 0.54

16.17 ± 0.38

17.61 ± 0.58

  1. Data shown is averages of replicates ± SD, n = 5.
  2. SFA saturated fatty acids, MUFA monounsaturated fatty acids, PUFA polyunsaturated fatty acids.
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