Table 4 The relative content (%) of fatty acids from Chlorella vulgaris extracted in dichloromethane/methanol (2:1 v/v; DCM/MeOH), hexane/isopropanol (2:1 v/v; hexane/IPA) and hexane, using three pre-treatments; no treatment (control), shaking at 1000 RPM for 60 min, (mixing), or sonication at 20 kHz for 10 min (sonication).
C. vulgaris | DCM/MeOH | Hexane/IPA | Hexane | ||||||
|---|---|---|---|---|---|---|---|---|---|
FA | Control | Mixing | Sonication | Control | Mixing | Sonication | Control | Mixing | Sonication |
C16:0 | 24.79 ± 0.55 | 24.91 ± 0.17 | 23.25 ± 0.08 | 30.03 ± 5.95 | 25.42 ± 0.03 | 27.41 ± 2.75 | 24.63 ± 0.6 | 26.69 ± 0.2 | 29.29 ± 5.05 |
C16:3n-4 | 10.40 ± 0.21 | 10.61 ± 0.07 | 10.80 ± 0.08 | 9.93 ± 1.32 | 10.62 ± 0.25 | 9.79 ± 0.65 | 12.18 ± 0.21 | 10.80 ± 0.24 | 8.95 ± 0.99 |
C18:0 | 1.49 ± 0.09 | 2.01 ± 0.10 | 1.49 ± 0.12 | 2.47 ± 0.56 | 2.86 ± 0.28 | 1.95 ± 0.15 | 6.11 ± 0.13 | 8.30 ± 0.11 | 2.80 ± 0.61 |
C18:1n-9 | 4.01 ± 0.12 | 3.96 ± 0.06 | 3.96 ± 0.04 | 3.47 ± 0.07 | 3.74 ± 0.08 | 4.02 ± 0.05 | 3.63 ± 0.01 | 4.46 ± 0.08 | 4.85 ± 0.15 |
C18:1n-7 | 1.10 ± 0.01 | 1.09 ± 0.01 | 1.04 ± 0.01 | 1.00 ± 0.03 | 1.10 ± 0.02 | 1.05 ± 0.01 | 0 | 0.92 ± 0.01 | 1.07 ± 0.04 |
C18:2n-6 | 37.34 ± 0.19 | 36.51 ± 0.02 | 37.53 ± 0.07 | 33.89 ± 3.02 | 35.29 ± 0.2 | 35.63 ± 1.27 | 32.93 ± 0.22 | 30.00 ± 0.04 | 33.88 ± 2.7 |
C18:3n-3 | 20.87 ± 0.25 | 20.90 ± 0.09 | 21.93 ± 0.03 | 19.21 ± 2.15 | 20.97 ± 0.25 | 20.15 ± 1.02 | 20.52 ± 0.31 | 18.83 ± 0.04 | 19.16 ± 1.65 |
SFA | 26.28 ± 0.60 | 26.92 ± 0.27 | 24.74 ± 0.16 | 32.50 ± 6.50 | 28.28 ± 0.57 | 29.36 ± 2.88 | 30.74 ± 0.73 | 34.99 ± 0.32 | 32.10 ± 5.52 |
MUFA | 5.11 ± 0.13 | 5.06 ± 0.05 | 5.00 ± 0.05 | 4.47 ± 0.09 | 4.84 ± 0.10 | 5.07 ± 0.06 | 3.63 ± 0.01 | 5.38 ± 0.10 | 5.92 ± 0.19 |
PUFA | 68.61 ± 0.61 | 68.02 ± 0.25 | 70.27 ± 0.14 | 63.03 ± 6.47 | 66.88 ± 0.65 | 65.57 ± 2.92 | 65.63 ± 0.74 | 59.63 ± 0.23 | 61.99 ± 5.34 |
