Figure 5

BDNF-mediated augmentation of GluN2B expressions contributes to the activation of silent synapses in HTP-GTE-treated OVX rats. (a–h) Failure rates for EPSCs at -70 mV or + 40 mV in slices from sham-OVX resilient + exo-vehicle, OVX helpless + exo-vehicle, OVX helpless + exo-BDNF, OVX helpless + exo-BDNF + exo-cyclo B, OVX helpless + exo-BDNF + ifenprodil, OVX helpless + exo-HTP-GTE, OVX helpless + exo-HTP-GTE + exo-cyclo B, and OVX helpless + exo-HTP-GTE + exo-ifenprodil. (i,j) Summary of the percentage of silent synapse for all groups (Sham-OVX resilient + exo-vehicle: 33.19 \(\pm \) 3.38, n = 7; OVX helpless + exo-vehicle: 3.93 \(\pm \) 3.89, n = 6; OVX helpless + exo-BDNF: 31.79 \(\pm \) 3.80, n = 6; OVX helpless + exo-BDNF + exo-cyclo B: 1.08 \(\pm \) 3.88, n = 7; OVX helpless + exo-BDNF + exo-ifenprodil: 5.52 \(\pm \) 5.12, n = 7; OVX helpless + exo-HTP-GTE: 44.61 \(\pm \) 7.64, n = 6; OVX helpless + exo-HTP-GTE + exo-cyclo B: 9.30 \(\pm \) 2.93, n = 7; OVX helpless + exo-HTP-GTE + exo-ifenprodil: 7.18 \(\pm \) 4.28, n = 7). (k–n) Representative traces for the effect of infeprodil on the NMDA EPSCs in hippocampal slices from sham-OVX resilient + exo-vehicle, OVX helpless + exo-vehicle, OVX helpless + exo-HTP-GTE, and OVX helpless + exo-BDNF groups. o. Percentage of ifenprodil-sensitivity current for all groups (Sham-OVX resilient + exo-vehicle: 50.62 \(\pm \) 2.91, n = 6; OVX helpless + exo-vehicle: 15.68 \(\pm \) 2.77, n = 5; OVX helpless + exo-HTP-GTE 40 μg/ml: 39.61 \(\pm \) 3.35, n = 6; OVX helpless + exo-BDNF 8 nM: 34.11 \(\pm \) 4.26, n = 5).