Figure 6

BDNF-mediated augmentation of GluN2B expression contributes to activation of silent synapses in HTP-GTE-treated OVX rats. (a,b) Western blot of GluN1, GluN2A, GluN2B levels in brain lysate for total and synaptoneurosomal fraction from hippocampus. Full-length blots are presented in Supplementary Fig. S13. (c–e) Quantification of GluN1, GluN2A, GluN2B levels (GluN2B, total: Sham-OVX resilient + exo-vehicle: 1.00 \(\pm \) 0.04; OVX helpless + exo-vehicle: 0.69 \(\pm \) 0.03; OVX helpless + exo-BDNF: 0.98 \(\pm \) 0.04; OVX helpless + exo-BDNF + exo-cyclo B: 0.66 \(\pm \) 0.07; OVX helpless + exo-HTP-GTE: 0.98 \(\pm \) 0.04; OVX helpless + exo-HTP-GTE + exo-cyclo B: 0.61 \(\pm \) 0.09; Synaptoneurosome: Sham-OVX resilient + exo-vehicle: 1.00 \(\pm \) 0.09; OVX helpless + exo-vehicle: 0.66 \(\pm \) 0.03; OVX helpless + exo-BDNF: 1.04 \(\pm \) 0.09; OVX helpless + exo-BDNF + exo-cyclo B: 0.66 \(\pm \) 0.07; OVX helpless + exo-HTP-GTE: 1.10 \(\pm \) 0.04; OVX helpless + exo-HTP-GTE + cyclo B: 0.66 \(\pm \) 0.04, n = 3 brains/group). (f,g) Western blot of GluN2B levels in brain lysate for total and synaptoneurosomal fraction from hippocampus. Full-length blots are presented in Supplementary Fig. S13. (h) Quantification of GluN2B levels (GluN2B, total: Sham-OVX resilient + exo-vehicle: 1.00 \(\pm \) 0.13; OVX helpless + exo-vehicle: 0.51 \(\pm \) 0.09; OVX helpless + exo-BDNF: 1.13 \(\pm \) 0.02; OVX helpless + exo-BDNF + exo-ifenprodil: 1.11 \(\pm \) 0.04; OVX helpless + exo-HTP-GTE: 1.14 \(\pm \) 0.04; OVX helpless + exo-HTP-GTE + ifenprodil: 1.11 \(\pm \) 0.03; Synaptoneurosome: Sham-OVX resilient + exo-vehicle: 1.00 \(\pm \) 0.02; OVX: 0.37 \(\pm \) 0.02; OVX helpless + exo-BDNF: 1.01 \(\pm \) 0.01; OVX helpless + exo-BDNF + exo-ifenprodil: 1.01 \(\pm \) 0.03; OVX helpless + exo-HTP-GTE: 1.08 \(\pm \) 0.05; OVX helpless + exo-HTP-GTE + exo-ifenprodil: 1.05 \(\pm \) 0.07, n = 3 brains/group). (i,j) Quantitative real-time PCR. Data represent the BDNF and GluN2B gene expressions normalized to GAPDH gene. Values are mean \(\pm \) SEM of three independent experiments. Data are represented as mean \(\pm \) SEM (One-way ANOVA Tukey’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001).