Figure 2 | Scientific Reports

Figure 2

From: Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes

Figure 2

Translocation of the p65 subunit of NF-kB to the nucleus of U87MG cells after LPS treatment. (A) Immunofluorescence confocal images at three different time points (0, 0.5, 12 h) of U87MG cells treated with LPS showed p65 translocation to nucleus after 12 h of treatment. Cells were incubated with anti-p65, followed by a secondary antibody anti-rabbit-Alexa Fluor 488, in green, and with DAPI, in blue. 400 × magnification (B) Western blot analysis of p65 with nuclear (Nu) and cytoplasmic (Cy) extracts of U87MG cells after 2 and 12 h of LPS treatment. Histone H3 was used as a loading control for nuclear extract. (C) Quantification of nuclear fluorescence intensity of p65 staining (n = 30) at the three time points (*p < 0.05 compared to 0 h and **p < 0.05 compared to 0.5 h by one-way Anova, Tukey test).

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