Figure 3 | Scientific Reports

Figure 3

From: Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes

Figure 3

Canonical and non-canonical gene expression profile after LPS stimulation of U87MG cells and of TCGA astrocytoma RNASeq data. (A) MYD88, TRAF6, JUN, SRF, IL1B, TRIF, NLRP3, RIPK1, RIPK3 expression ratio with the non-treated cell were accessed by qRT-PCR, at different time points (0.5, 12, 24, 48 h) in three independent experiments. The fold change values were calculated by the ratio of the value obtained by 2−ΔCt formula of treated cells compared to control cells (time point 0). (B) Heatmap of the RPKM values from the TCGA RNASeq dataset, normalized by z-score for the selected genes of astrocytoma cases of different malignant grades (AGII, AGIII, and GBM). GBM cases were subdivided by molecular subtypes proneural (PN), classical (CS), and mesenchymal (MES). Upregulated values are in red and downregulated in blue.

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