Figure 5 | Scientific Reports

Figure 5

From: Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes

Figure 5

TLR4 localization in U87MG cells. (A) TLR4 presence was observed in nuclear (Nu) and cytoplasmic (Cy) protein fractions of U87MG cell line by Western blot analysis. Total H3 protein was used for nuclear protein enrichment control and β-actin for protein loading control. (B) The presence of TLR4 was detected in cytoplasmic and nuclear compartments in U87MG cells by immunofluorescence. TLR4 staining in the nucleus increased after 12 h of LPS stimulation. Cells incubated with anti-TLR4-secondary antibody anti-mouse-Alexa Fluor 568, in red, and with DAPI, in blue. 200 × magnification. (C) Values for nuclear fluorescence intensity for each analyzed cell n = 23 at 0 h and n = 25 at 12 h (***p < 0.001 compared to 0 h, Mann-–Whitney).

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