Figure 2
From: DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
Nitric oxide liberation from chicken HD11 macrophage cell line over increased concentrations of RCA products ((A) RCA I to RCA IV in comparison with NC-RCA, (B) RCA I in comparison with NC-RCA and anti-chicken CD40 MAb). The RCA products were incubated overnight with the HD11 macrophage cell line in concentrations 1, 5, 10, 25, 50, 100, 200, 300 and 350 (Ī¼g/mL) in triplicates. The nitric oxide liberated was measured using Griess assay and showed slop increasing in regard to dose applied. The result was compared to anti-chicken CD40 mAb which is known to have the effect on this cell line (Chen et al., 2010) and negative control RCA (two candidates randomly picked from the library and was not enriched in the Illumina sequencing results). Multiple way ANOVA and Tukey honest significant difference test (HSD) were applied to detect significant differences within treatments. Different uppercase letters represent difference at pā<ā0.0001.
