Figure 3
From: DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
LC–ESI–MS results. After the incubation of the chCD40ED protein (25,712 Dalton) with the aptamers sequences (SEQ3, SEQ4, SEQ3 + SEQ4 or NC-SEQ), the protein was subjected to the action of trypsin digestion for overnight. Three distinct regions (Regions 1, Region 2 and 3 in (A), (B) and (C), respectively; see also Fig. 4B) on the protein sequence were protected from the digestion with trypsin enzymes (A,B and C), mostly by SEQ3 + SEQ4. The data collected at different time point (s) were plotted to show the intensity of the peaks. Other regions in Figure (C), (D) where little protection is provided by aptamers are also shown as a negative control.
