Figure 4
From: DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
Virtual tertiary structure of chCD40ED showing the potential aptamers linking sites. PyMOL 2.2 software (https://pymol.org/2/) was used for modeling and visualization. (A) Cartoon modeling shows the tertiary structure of the protein. (B) Space-filling model representing the virtual aptamers-protein interaction sites. Three regions (blue, yellow and orange) determined by MALD-TOF–MS experiment. The blue region represents long flabby peptide sequence where CD40L attach. (C) Top view of the 3D structure of the chCD40ED. This model shows virtual trimer configuration of CD40 receptor (Mesh model, gray) with the potential aptamer linking sites (space-filling model, blue, white and black). These sites located in the inner of the receptor resembling the actual ligand binding sites (see below).
