Figure 5

Normalized ELISA results representing S–N values of the anti-M2e IgG in chicken sera at weekly intervals after immunization. Birds (n = 20) were immunized at day 7 and day 21 via s/c route with (1) None (negative control), (2) M2e peptide, (3) aptamer RCA II, (4) mAb-SA-M2e, (5) aptamer RCA II-SA-M2e complex at 25 μg/bird or 50 μg/bird. The normalization calculations S–N = (any read—mean reads of the non-immunized group). The mean of each group calculated accordingly. Results show mean ± SE and the differences are according to ANOVA and student t-test. Asterisks represent statistical differences (*p = 0.0001) from the non-immunized group.