Figure 2
Microscopic analysis of GUS expression patterns in root-knot nematode-inoculated tomato roots harboring LOX1.2, AOS1, OPR2 and, α-DOX1 promoter: GUS fusion constructs. (A1–E1) Micrographs of LOX1.2–GUS reporter line. (A1) Noninoculated root harboring the LOX1.2:GUS fusion construct exhibits no GUS signal related to root tip or elongation zone. (B1) Roots 2 days after inoculation (DAI). (C1) Inoculated roots 5 DAI. (D1) Developing galls 15 DAI. (E1) Mature galls 28 DAI. (A2–E2) Micrographs of AOS1–GUS reporter line. (A2) Noninoculated root harboring the AOS1:GUS fusion construct exhibits no GUS signal related to root tip or elongation zone. (B2) Roots 2 DAI. (C2) Inoculated roots 5 DAI. (D2) Developing galls 15 DAI. (E2) Mature galls 28 DAI. (A3–E3) Micrographs of OPR2–GUS reporter line. (A3) Noninoculated root harboring the OPR2:GUS fusion construct exhibits no GUS signal related to root tip or elongation zone. (B3) Roots 2 DAI. (C3) Inoculated roots 5 DAI. (D3) Developing galls 15 DAI. (E3) Mature galls 28 DAI. A5–E5, Micrographs of αDOX1–GUS reporter line. (A4) Noninoculated root harboring the αDOX1:GUS fusion construct exhibits no GUS signal related to root growth. (B4) Roots 2 DAI. (C4) Inoculated roots 5 DAI. (D4) Developing galls 15 DAI. (E4) Mature galls 28 DAI. (A5–E5) Micrographs of pCAMBIA2300–GUS reporter line; empty pCAMBIA2300 vector fused to GUS reporter served as a control. (A5) Noninoculated root harboring the pCAMBIA2300:GUS fusion construct exhibits no GUS signal related to root tip or elongation zone. (B5) Roots 2 DAI. C5 Inoculated roots 5 DAI. (D5) Developing galls 15 DAI. (E5) Mature galls 28 DAI. Arrows indicate GUS staining (C1, D2, E2, E3, D4 and E4): Micrographs viewed under a light microscope. Bright-field image of roots and galls photographed through a stereomicroscope. Bars: A1–A5, B1, B4, B5, C1, C4–C5, D3–D5 = 50 μm; B2–B3, C2–C3, D1–D2, E1–E5 = 500 μm.
