Figure 4
(A) Venn diagram depicting the distribution of DEG including Signal Peptide according to SignalP5.0. (A.1) Venn diagram showing the number of overlapping and nonoverlapping total differentially expressed genes of M. javanica transcripts following exposure to: protoplasts/control (Cont.) and 9-HOT/control. (A.2) Distribution of up-regulated genes found in each treatment as well as genes overlapping between both treatments. (A.3) Distribution of down-regulated genes found in each treatment as well as genes overlapping between both treatments. Fold change with an absolute value > 2 and < − 2 and p value ≤ 0.001 was used for the analyses. (B) Gene ontology (GO) annotations of differentially expressed genes including Signal Peptide according to SignalP5.0, of M. javanica J2 exposed to 9-HOT vs. control unigenes at multilevel using BLAST2go software. The GO terms were categorized into (B.1) biological process and (B.2) molecular function. Pie chart slices represent the percentages of unigenes identified in a particular category among the differentially expressed genes encoding effectors.
