Figure 5
FISH of dissected freshly hatched M. javanica J2. M. javanica J2 were stained with DAPI (blue) together with (A) Triacylglycerol lipase Cy5-specific probe (red). (A1–4) M. javanica J2 exposed to 9-HOT. Esophageal gland area: D, dorsal gland; SV, subventral glands (combined Z sections). (A5–8) M. javanica exposed to 0.01 M MES buffer + ethanol; EG, esophageal gland region. 1 and 5 DAPI-stained nuclei (blue) of the dissected nematode as seen from combined sections under fluorescence. 2 and 6 FISH signal of triacylglycerol lipase Cy5-specific probe (red) as seen from combined sections under fluorescence. 3 and 7 Combined DAPI-stained nuclei (blue) and FISH signal (red) under bright field. 4 and 8 M. javanica as seen from combined sections under bright field and fluorescence. (B) MLT-10 like Cy5-specific probe (red). (B1–4) M. javanica J2 exposed to 9-HOT. SV, subventral glands (combined Z sections). (B5–8) M. javanica exposed to 0.01 M MES buffer + ethanol; EG, esophageal gland region. 1 and 5 DAPI-stained nuclei (blue) of the dissected nematode as seen from combined sections under fluorescence. 2 and 6 FISH signal of MLT-10 Cy5-specific probe (red) as seen from combined sections under fluorescence. 3 and 7 Combined DAPI-stained nuclei (blue) and FISH signal (red) under bright field. 4 and 8 M. javanica as seen from combined sections under bright field and fluorescence.
